Improved Glutamic Acid Production Capacity of Corynebacterium glutamicum by the ARTP Mutagenesis Method
نویسندگان
چکیده
Glutamic acid is an important amino that used widely in the fields of food, medicine, and agriculture. One methods glutamic production direct microbial fermentation, so genetic stability glutamic-acid-producing capacity producing strain are keys to improving concentration. Experiments were carried out using Corynebacterium glutamicum GL−6 as parental strain, with two iterations mutagenesis by atmospheric room temperature plasma (ARTP) screening agar plates tolerant high sugar malonic acid, best strains stable phenotypes verified fermentation 20 L tanks. The results show optimal time ARTP was 140 s, lethality positive mutation rates 93.0% 15.6%, respectively. concentrations high-sugar 240 g/L 35 g/L, A mutant P−45, improved stability, obtained through rounds iterative screening. concentration this Erlenmeyer flasks 17.7 which 18.8% higher than GL−6, could be inherited stably for 10 generations. In synthesis pathway, upregulation gene encoding citrate synthase (cs), isocitrate dehydrogenase (icdh), glutamate (gdh), downregulation oxoglutarate complex (odhc) increased carbon flows TCA cycle its branch metabolic flow synthesis. P−45 showed a 147.0 under fed-batch conditions tanks, 81.5% starting GL−6. This study provides new technical solution metabolites stability.
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ژورنال
عنوان ژورنال: Fermentation
سال: 2023
ISSN: ['2311-5637']
DOI: https://doi.org/10.3390/fermentation9070599